Today at #IALSJanelia, we're exploring super-resolution. This includes spending time at @aicjanelia.bsky.social's iPALM system, where participant are exploring tubulin networks (purples) and focal adhesions (light blue) at the ~20 nm scale! iPALM data by @anjanja1024.bsky.social

Lattice light sheet data taken by the participants of #IALSJanelia 2026, with guidance from @cmhobson.bsky.social. I rendered the actin dynamics using #AGAVE 🔬

You have to love the diversity of shapes that these HL60 cells make! #IALSJanelia 2026

The tools in OpticalFlow3D are applicable across many length scales, including whole organisms! Measurements of dorsal-ventral motion highlight folding during Drosophila embryogenesis. (4/7)

OpticalFlow3D is designed to apply a Lucas-Kanade-based flow algorithm to 3D images, revealing complex biological motion. Here, flow highlights formation of a contractile actin ring in a dividing cell -- dark blues indicate less motion, while yellow highlights regions of high actin activity. (3/7)

Optical flow measures spatial and temporal changes in intensity, rather than the motion of single object. This allows it to quantify myosin II motion even when the fibers are difficult to segment. In this movie, blue indicates outward motion, while red indicates inward motion. (2/7)

Yes, they are the same field of view 😄 Bonus additional #InsightFromImaging cell from a different FOV here:

HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social

When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃

Today marks 4 years of enjoying the beautiful @hhmijanelia.bsky.social campus 🌳

April is #CitizenScienceMonth! There is still time to join @scistarter.bsky.social and be part of #OneMillionActsofScience 2025. I had fun observing some trees for the Globe Observer program 🌳

Many thanks to the @hhmijanelia.bsky.social Lab Coordinators for another wonderful Cookie Party! The @aicjanelia.bsky.social / @i2janelia.bsky.social team had a ton of fun! Photo featuring: @desantis42.bsky.social, @mairahmoon.bsky.social, @harikrushnan.bsky.social, @anjanja1024.bsky.social

As a hello to Bluesky, here is some blue sky over the Janelia Research Campus 😊