Bio-protocol
@bio-protocol
Bio-protocol (www.bio-protocol.org) was founded in 2011 by a group of researchers at Stanford University with a singular mission: Improving research reproducibility by publishing high-quality protocols in the life sciences.
Bio-protocol's new issue is now live! 🎉https://bio-protocol.org/cn/archive?vol=16&issid=1401
A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar Work by Guoqian Yang, Yang Yu, Vijaya Kumar Reddy Vulavala, Nidhi Dwivedi and Chang-Jun Liu at Brookhaven National Laboratory. #LifeSciences #Reproducibility #PlantScience
Efficiency-Corrected Relative Quantification of qPCR Data Using LinRegPCR and a Spreadsheet-Based Workflow Work by Louis Arnould Müller and Laurent Tiret at Ecole nationale vétérinaire d’Alfort. #LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Protocol for In Vivo Two-Photon FCS to Measure Nanocarrier Number and Flow Velocity in Mouse Cerebral Microvasculature Work by Sagar Pande, Xiaojin Wang, Xu Fu, Harini Prathapasinghe and Christopher I. Richards at University of Kentucky. #LifeSciences #Reproducibility #Neuroscience
Stepwise Differentiation of Mouse Embryonic Stem Cells Into Murine Blood Vessel Organoids With Endothelial Lineage Tracing for Quality Control Work by Sophie Guelfi, Sarah Bopp and Gabriele Bergers at VIB-KU Leuven Center for Cancer Biology. #LifeSciences #Reproducibility #StemCell
An Immunoprecipitation-Based Nonradioactive Kinase Assay to Measure Akt Kinase Activity in Mammalian Cell Lines Work by Amber Peek, Jay N. Mehta and Deepali Bhandari at California State University, Long Beach. #LifeSciences #Reproducibility #Biochemistry
Construction and Functional Evaluation of Cyclic Peptide-Based CAR T Cells in Tumor Model Work by Xiaoting Meng, Qingmin Wu and Yu-Hsuan Tsai at Shenzhen Bay Laboratory. #LifeSciences #Reproducibility #CancerBiology
📢 Bio-protocol Webinars present: Engineering next-generation kidney organoids: Enhancing physiological relevance, reproducibility, and scalability 🔗 Register here: bio-protocol.org/register/web...
Whole-Mount Immunostaining of Tyrosine Hydroxylase for Dopaminergic Neuron Analysis in Zebrafish Larvae Work by Luís Félix at University of Trás-os-Montes and Alto Douro. #LifeSciences #Reproducibility #Neuroscience
Generation and Characterization of Adaptive Anoikis-Resistant Cells Using Cyclic Attachment-Detachment Culture of Cancer Cells Work by Resha Rajkarnikar, Mehri Monavarian and Karthikeyan Mythreye at The University of Alabama. #LifeSciences #Reproducibility #CancerBiology
Multiply Perturbed Response: A Computational Protocol to Identify Cooperative Allosteric Residue Combinations Driving Protein Conformational Transitions: bio-protocol.org/en/bpdetail?...
An Optimized Protocol for the Characterization of Zebrafish ApoB-Containing Lipoproteins Using the LipoGlo System Work by Monica R. Hensley and Steven A. Farber at Johns Hopkins University. #LifeSciences #Reproducibility #Biochemistry
Bio-protocol's new issue is now live! 🎉 bio-protocol.org/en/archive?v...
Electrophoretic Mobility Shift Assay (EMSA) for Assessing RNA–Protein Binding and Complex Formation Using Recombinant RNA-Binding Proteins and In Vitro–Transcribed RNA: bio-protocol.org/en/bpdetail?...
DiRT v2.0: An Optimized Pipeline for Detecting Dicistronic tRNA-mRNA Transcripts in Plants: bio-protocol.org/en/bpdetail?...
Amplification-Free Detection of Highly Structured RNA Molecules Using SCas12aV2:https://bio-protocol.org/en/bpdetail?id=5462&type=0&source=Bluesky
Visualizing Diverse RNA Functions in Living Cells With Spinach(TM) Family of Fluorogenic Aptamers Work by Ryan O’Hanlon and Karen Y. Wu at Lucerna. #LifeSciences #Reproducibility #MolecularBiology
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Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture Work by Eleanor I. Lamont, Richard M. Jones, Jessica Assadi, Shuyi Ma and David R. Sherman at University of Washington. #LifeSciences #Reproducibility #Microbiology
Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data Work by Maya N. Walker, Bo Hu, Shi-Yuan Cheng and Xiao Song at Northwestern University Feinberg School of Medicine. #LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications Work by Jenni K. Pedor, Pavlina Gregorova, Salla M. Kalaniemi and L. Peter Sarin at University of Helsinki. #LifeSciences #Reproducibility #Biochemistry
Simultaneous Immunofluorescence-Based In Situ mRNA Expression and Protein Detection in Bone Marrow Biopsy Samples Work by Alba Lillo Sierras, Sandro Bräunig, Hongzhe Li and Stefan Scheding at Lund University. #LifeSciences #Reproducibility #Biochemistry
Bio-protocol's new issue is now live! 🎉 bio-protocol.org/cn/archive?v... This issue features the special collection “Technologies for RNA Detection,” highlighting advances in RNA detection and visualization, sequencing, structural analysis, and RNA–protein interaction studies.
Histomorphometrical Analyses of the Mouse Suprachiasmatic Nucleus: bio-protocol.org/en/bpdetail?...
Computational Quantification of Mouse Retinal Vasculature Using ImageJ Work by Michel Nader, Hirad A. Feridooni, Mahtab Tavasoli, Sarah van der Ende, Christopher R. McMaster, and Johane M. Robitaille at Dalhousie University. #LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
ROOT-ExM: Super-Resolution Imaging of Proteins in Arabidopsis Roots by Expansion Microscopy: bio-protocol.org/en/bpdetail?...
Evaluating Thioredoxin-Mediated CF(o)CF(1)Reduction Using an In Vitro Thylakoid Assay Work by Takatoshi Sekiguchi, Keisuke Yoshida and Toru Hisabori at Tokyo Institute of Technology. #LifeSciences #Reproducibility #PlantScience
📢 Reminder: Don't miss our upcoming webinar, 'Visualizing diverse RNA functions in living cells with the Spinach™ family of fluorogenic aptamers'. 🗓 Date: Jun 18, 2026 ⏰ Time: 11:00 AM ET | 8:00 AM PT
Detection of Target Molecules Within One-Millimeter-Thick Mouse Brain Slices by Using Peroxidase-Fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Reaction: bio-protocol.org/en/bpdetail?...
Quantitative Analysis of Cell and Tissue Shape During Mouse Cranial Neural Tube Closure Work by Kristina A. Borys and Eric R. Brooks at North Carolina State University. #LifeSciences #Reproducibility #DevelopmentalBiology