David Monchaud
@dmonchaud
🥖😬👨🎨 (France) - 🍯🍷🌧️ (Dijon) - 🧬🔬🧪 (CNRS) Chemical biology/genetics. Occasional fiction writer in the grant-proposal genre. Trying to understand why and how DNA and RNA fold into ωєιя∂ℓу ѕнαρє∂ structures.
We keep on investigating the role(s) of G-quadruplex #G4 in aging and found a quite interesting correlation between age and the level of both G4s and related G4 helicase (particuarly here: DDX3X). With @mcgovernpeds.bsky.social In Cell Death Dis. @springernature.com 👉 doi.org/10.1038/s414...
If you're working with guanine (G)-rich DNA/RNA sequences and want to know whether they fold into G-quadruplex (G4) structures—and which topology they adopt—it can now be done with the naked eye.. or almost! 🔬 Now in @sciencechinapress.bsky.social @springernature.com 👉 doi.org/10.1007/s114...
And many thanks to Juan Carlos for the tour around Granada (and for the famous tapas!) 🇪🇸
With the goal of further developing our #G4 molecular helicase 𝗣𝗵𝗽𝗖, we—in collaboration with Carla Cruz @ubeirainterior.bsky.social—have investigated the biodistribution of this molecule using gold nanocarriers for controlled release. Now in JDDST @elsevierlsj.bsky.social 👉 doi.org/10.1016/j.jd...
In Genome Biol. @springernature.com we report on: 1/ the 𝗮𝗻𝘁𝗶𝗰𝗮𝗻𝗰𝗲𝗿 𝗮𝗰𝘁𝗶𝘃𝗶𝘁𝘆 of the G-quadruplex #G4 stabilizer 𝗤𝗡-𝟯𝟬𝟮 and a 𝘀𝘆𝗻𝘁𝗵𝗲𝘁𝗶𝗰 𝗹𝗲𝘁𝗵𝗮𝗹𝗶𝘁𝘆 𝘀𝘁𝗿𝗮𝘁𝗲𝗴𝘆 to improve it, and 2/ the genoprotective role of the G4 unwinder 𝗣𝗵𝗽𝗖 With @ucllifesciences.bsky.social & @westernu.ca 👉 doi.org/10.1186/s130...
Happy to take you behind the scenes of the discovery of 7 alternative #DNA structures: 𝘁𝗿𝗶𝗽𝗹𝗲𝘅, G-quadruplex (𝗚𝟰), 𝗶-𝗺𝗼𝘁𝗶𝗳, both 3- and 4-way 𝗗𝗡𝗔 𝗷𝘂𝗻𝗰𝘁𝗶𝗼𝗻𝘀, 𝗥-𝗹𝗼𝗼𝗽 and the left-handed duplex 𝗭-𝗗𝗡𝗔. Hope you'll enjoy this journey back in time! In @narjournal.bsky.social 👉 doi.org/10.1093/nar/...
It was super great to welcome Chun Kit Kwok, from CityUHK, for a 2-day visit in our lab. A very enjoyable time discussing science in general, with of course a deep dive into RNA and G-quadruplex #G4 RNA. Thank you for coming!
Biocatalysts made of DNA, and G-quadruplex #G4 DNA in particular, have found a myriad of applications. But is there a real-life application reported so far? Thrilled to report this long-awaited example with the remediation of industrial wastewater! In @academic.oup.com doi.org/10.1093/nsr/...
FYI: the molecular helicase PhpC we developped to unfold G-quadruplex #G4 DNA and RNA both in vitro (see: shorturl.at/Ep8Il) and in human cells (e.g., shorturl.at/ue7rV, shorturl.at/zsheP & shorturl.at/MfUKW) is now commercially available at Idylle @idylle-labs.bsky.social 👉 shorturl.at/efE5l
Happy to be one of these guys going down the White Dune before swimming 10k across the Dakhla bay. 4 days, 4 stages, 4 adventures. Thank you Morocco Swim Trek 🙏 👉 moroccoswimtrek.com
How to recapitulate in vitro what happens in cells? Not easy! We investigated how guanine (G)-rich sequences interact with one another to decipher G4-associated gene expression regulation With Nanjing U & @polytechniqueparis.bsky.social @cnrs.fr In @narjournal.bsky.social: doi.org/10.1093/nar/...
A huge thank you to Jean-Louis Mergny, Jérome and Pascale @polytechniqueparis.bsky.social for organizing the "G-quadruplex (G4) made in France": two days filled with diverse and fascinating exchanges on the many facets of #G4 research!
Super proud of my team! They have simplified the synthesis of TASQs, enabling easier & faster access to these biomimetic G-quadruplex (G4) ligands used to image and identify cellular G4s. The TASQs story: doi.org/10.1021/acs.... This new article @chemistryeurope.bsky.social: doi.org/10.1002/chem...
A novel biotechnological application for G4s, used for controlling the activity of a CRISPR-Cas12a device used for diagnosing human viral infection (HPV) in a spatio-temporal manner Now in Anal. Chem. @pubs.acs.org With Nanjing U. & @polytechniqueparis.bsky.social @cnrs.fr doi.org/10.1021/acs....
The photoMultiTASQ is a new molecular tool to investigate G-quadruplex #G4 biology, notably for identifying G4 binding proteins by Chem-CLIP (chemical cross-linking and isolation by pull-down) In ACS Bio & Med Chem Au: doi.org/10.1021/acsb... With @iphc-strasbourg.bsky.social (FR) & WIS Rehovot (IS)
Stimulating scientific days #ChemBioParis2025. Super great to hear Shankar Balasubramanian speaking about G-quadruplex, Matthew Disney about ChemCLIP and Dan Nomura about MYC druggability, among many others Kudos to @borisvauzeilles.bsky.social & the team for the organization chembioparis2025.com
Honored to have been interviewed by Pour La Science, a wide-audience scientific magazine, to discuss the results from @simonelsasser.bsky.social / @puckknipscheer.bsky.social published in @science.org about how DNA repair proteins mold G-quadruplex #G4 👉 tinyurl.com/3k6pxu6d (sorry, in French only)
Happy to describe a novel strategy to detect pollutants in food with a super high sensitivity, using G-quadruplex #G4 based biosensing devices. A collaboration with the University of Nanjing (CN) and Polytechnique Palaiseau (FR) Now in Anal. Chem. (@pubs.acs.org): doi.org/10.1021/acs....
A novel demonstration of the G-quadruplex #G4 unfolding properties of PhpC, a quite unique prototype of G4 unfolder (or molecular helicase) we developed in the lab In collaboration with C. Cruz @ubeirainterior.bsky.social and R.H.E. Huson @westernu.bsky.social 👉 doi.org/10.1016/j.ij...
The best of the best! The core of our community on #DNA #RNA junctions during the first meeting #IMAJ1 including @biometalusc.bsky.social, @sebastien-britton.bsky.social, @christinecardin.bsky.social, @granzhan.bsky.social, Jean-Louis, Mark and many others Thank you again for your participation!
It was an absolute thrill and a real pleasure to organize the first meeting on #DNA #RNA junctions in Paris. Fantastic talks, lively discussions—and some excellent wine too! 🍷😁 A very promising beginning for this new community Stay tuned—we’ll be back next year! #IMAJ1 #CNRSchimie @cnrs.fr
We're thrilled to have contributed to this @naturecomms.bsky.social paper on the role of G-quadruplex #G4 in mitochondrial RNA G4s can be targeted with ligands to inhibit mitochondrial translation and eventually functions, which jeopardizes cancer cells fate This is huge! 👉 doi.org/10.1038/s414...
Welcome to BlueSky @jamchemsoc.bsky.social ! Happy to celebrate your arrival amongst us with this new article (doi.org/10.1021/jacs...), in which we report on CoRP-seq, a technique for mapping RNA G-quadruplex #G4 transcriptome-wide in a fast, cheap and simple manner #chembio #AcademicSky #SciSky
A fully detailed protocol to visualize G-quadruplex #G4 DNA/RNA in human cells using clickable G4 ligands (in situ click imaging) now in @bio-protocol.bsky.social Interested?? All you need is: - the clickable ligand: www.sigmaaldrich.com/FR/en/produc... - and the protocol: doi.org/10.21769/Bio...
Quite happy to see this out in @chemistryeurope.bsky.social (with ENS Paris FR, Szeged University HU, UTC Prague CZ) We report here on molecular tools used to detect G-quadruplex #G4 DNA/RNA in vitro, and on the mechanism underlying their unique photophysical properties. 👉 doi.org/10.1002/cmdc...
A fully detailed method (40+ steps) to isolate/identify RNA G-quadruplex #G4 in human cells using G4-RNA precipitation (G4RP) protocol G4RP.v1 in @natureportfolio.bsky.social: doi.org/10.1038/s414... & nature.com/articles/s41... & G4RP.v2 now in @cellpressnews.bsky.social: doi.org/10.1016/j.xp...
One of the ones I am most proud of. In this article, we abided by the very definition of chemical biology #ChemBio to decipher the role that DNA/RNA G-quadruplex #G4 plays, and the effects that the G4 ligands have, in human cells. 👉https://dx.doi.org/10.1016/j.jbc.2024.108040 @cnrs.bsky.social