Hao Yin
@haoyin
Twitter/X @haoyin20 Vascular biologist
Robust decomposition of cell type mixtures in sequencing-based #SpatialTranscriptomics RCTD www.nature.com/articles/s41... Cell type-specific inference of differential expression in spatial transcriptomics C-SIDE www.nature.com/articles/s41... spacexr github.com/dmcable/spac...
Histone AcetylTransferases P300 & CBP have distinct functional roles in Arterial Smooth Muscle Cell phenotypes P300 pro-contractile, working with TET2 CBP pro-migratory, working with HADC2/5 Converging on H3K9/27ac-SRF-Myocardin axis #Circulation 2022 www.ahajournals.org/doi/10.1161/...
Lymphatic Endothelial Cell VEGFR2 in postnatal skin Lymphangiogenesis & Lymphatic valvulogenesis 🐭 Cdh5-CreERT2;Vegfr2 f/f (P1~2,P10~12) Prox1-CreERT2;Vegfr2 f/f (P1~4,P10~12) VEGFR2-deficient lymphatic ECs gain hematopoietic transcript traits #Angiogenesis 2026 link.springer.com/article/10.1...
Enzyme-free isolation of mouse brain/cortex vasculature for proteomics glass Dounce homogenizer 70 kDa dextran density-gradient centrifugation (you don't need ultracentrifugation) 30–80 μg total protein yield per adult mouse @rrust.bsky.social #STARProtocols 2026 www.cell.com/star-protoco...
Linked Inference of Genomic Experimental Relationships (LIGER) #MultiomicsIntegration Integrative nonnegative matrix factorization▶️ latent factor/metagenes github.com/welch-lab/li... #NatureProtocols 2020 www.nature.com/articles/s41...
A possible rejuvenating cocktail for replicatively senescent Endothelial Cell valproic acid HDACi lithium carbonate GSK3i Galunisertib ALK5i Transient modulation of Yamanaka factors Alleviating pro-inflamm SASP Restoring angiogenic behaviors #BasicResCardiol 2026 link.springer.com/article/10.1...
Bulk Transcriptome of Coarctation of the Aorta 8 case + 6 control Control: 4x dextro-transposition of the great arteries; 1x major aortopulmonary collateral arteries; 1x subvalvular pulmonary stenosis ⬆️Collagen matrix ⬆️HOX ⬆️Retinoic acid signaling #JCM_MDPI 2026 www.mdpi.com/2077-0383/15...
Induced Lox KO in Myh11+ Smooth Muscle Cell, but not PDGFRα+ fibroblast, ⏬Angiotensin II-induced 🐭Cardiac Fibrosis Cardiac hypertrophy remain unaltered in both Lox KO models A possible SMC-to-Fibroblast matrix/mechano-transducive cascade in fibrosis bioRxiv 2026 www.biorxiv.org/content/10.6...
Pathobiology of Aortic Aneurysms & Dissections Multiple hypothetical drivers for #AorticDissection 👉Cystic medial degeneration 👉Vasa vasorum dysfunction 👉Loss of intralamellar interconnecting elastic fibers in outer media #AortaEd #JACCAdv 2025 www.sciencedirect.com/science/arti...
MyoCarDin protein migrates at a MW of 130–140 kDa, not 100 kDa as predicted, partly due to constitutive phosphorylation Rat & Mouse with CRISPR HA-tagged MyoCD Nearly the entire Myocardin protein is predicted to be intrinsically disordered #AJPCellPhys 2026 journals.physiology.org/doi/full/10....
#AorticAging Sex-specific Aortic Geometric Atlas Centile-Based Reference Charts and Pathological Signatures Across the Adult Lifespan >63k TotalSegmentator Phenome-wide time-to-event analysis 155 phecodes x 28 aortic geometric phenotypes #AortaEd medRxiv 2026 www.medrxiv.org/content/10.6...
De novo Elastogenesis in Aortic False Lumen Wall 1⃣👤Type B #AorticDissection N=3 2⃣Surviving🐭+12-wk BAPN False lumen mural cells 👉High Elastin mRNA/lamina 👉60% ACTA2+MYH1+; 20% negative 👉Contributed by Gli+ adventitial progenitors, not pre-existing SMCs bioRxiv 2026 www.biorxiv.org/content/10.6...
#Pericyte #TunnelingNanoTube #Retinopathy #SciArt Pericyte number⬆️ but Interpericyte TNT⬇️ in Retina vasculature in rats +STZ 5 wk A nice spatial (3 layers & 4 quadrants) analysis Interpericyte TNT connects capillaries & venule/arteriole-capillary🥸 #IOVS 2026 iovs.arvojournals.org/article.aspx...
Nature News covered our preprint on LLM analysis of Preprint-to-Publication transition 😆👇 Think preprints are unreliable? Analysis of 70,000 studies might change your mind www.nature.com/articles/d41... Kudos to @rrust.bsky.social for leading this project👍 www.biorxiv.org/content/10.6...
Interested in Aging & Neurodegeneration & tackling them using a single-cell-defined organism🪱?😍 Do not miss this PostDoc opportunity at the Spaulding Lab, the MDI Biological Laboratory🤠👇
Aortic Ultimate Stress & Ultimate Stretch⬇️ in Type A #AorticDissection 19 case + 9 donor control Ex vivo uniaxial tensile test Both axial & circumferential aortic strip (30 x 3 mm) Adventitia removed #AortaEd #MedBiolEngComput 2026 link.springer.com/article/10.1...
#EndothelialCell #PlanarCellPolarity Mutated in Colorectal Cancer BioID interactome in EC▶️ Centriolar proteins +ROBO1 KEAP1 p62 EC MCC KO🐭Retina ⏬Vascularity ⏬Engaged sprouting tip cells (cell body orientation perpendicular to migration front) #Angiogenesis 2026 link.springer.com/article/10.1...
Protein Arginine Methyltransferase 5 as a negative epigenetic regulator of SRF/Myocardin-mediated contractile phenotype of Smooth Muscle Cell PRMT5 ⏫H3R8me2 H3R4me2 SMC Prmt5 KO🐭; Lenti-shPRMT5; EPZ015666 (PRMT5i)▶️ ⏬Neointima in injured carotid #CardiovascRes 2023 academic.oup.com/cardiovascre...
Take a look at our LLM analysis of the evolution of preprints into peer-reviewed forms😎 biorxiv.org/content/10.6... Thanks a million to @rrust.bsky.social for this inspiring project😆 No surprise ~90% preprints that pass peer reviews had minimal-to-minor revisions of their scientific claims🤠
Nidogen-2, a basement membrane component, directly binds to & activates Jag1 & Notch3 in Smooth Muscle Cell➡️⏫Contractile phenotype Nid2 KO🐭➡️⏫Neointima in wire-injured carotid artery Nidogen-2 ⬇️in SMC +PDGFBB ⬆️in SMC =TGFβ #Circulation 2021 www.ahajournals.org/doi/10.1161/...
In patients with Spontaneous cervical artery dissection (n=5), Capillary enlargement are observed in 40~56% capillary loops at finger tips (Nailfold capillaroscopy) #CaseReport #Angiogenesis 2026 link.springer.com/article/10.1...
Frequency of Thoracic Aortic Dilation in Noonan Syndrome #RASopathy N=37 0.6~32 y/o 14 PTPN11 variant carriers (out of 16 known variants) Aortic diameter Z > 2 10 Aortic annulus 8 Aortic Root 2 Ascending aorta #AortaEd #AmJCardiol 2014 www.sciencedirect.com/science/arti...
A 3D Microfluidic Smooth Muscle Cell Calcification Model Immortalized SMC + 4d calcifying medium Fibrin + VP-co-GMA copolymer Hydrogel Fetuin A-mRuby as a dye for calcium mineral particle Calcification can spread throughout the gel😍 #FEBSOpenBio 2026 febs.onlinelibrary.wiley.com/doi/10.1002/...
Sinusoidal endothelial cells control liver inflammation & fibrosis A very enjoyable piece on pathogenic liver #Angiocrine (CCL2 CXCL1) & transcriptional programs (GATA4 ZEB2 RUNX3 NOTCH) A novel BRD4/PML phase separation superenhancer trigger #JCI 2026 www.jci.org/articles/vie...
#FGF9 #LeydigCell In rats with dimethane sulfonate-induced Leydig cell depletion FGF9 ⏫Leydig cell prolifer/maturation ⏫Testosterone production +Evidence from 3D cultured seminiferous tubule FGF9 ⏫Leydig markers Scarb1 Stard1 Cyp11a1 Cyp17a1 #Reproduction 2026 academic.oup.com/reproduction...
Robo2 as a surface marker for FACS 👤dermal papilla cells Robo2 is induced by CHIR99021 + FGF9 CH+F9 seems superior to BIO+BMP2+FGF2 & synergize with 3D aggregation culture in maintaining hDPC markers (WNT5A/LEF1/NOG/BMP4/VCAN/SPRY4/SOX2) #ExpDermatol 2026 onlinelibrary.wiley.com/doi/10.1111/...
#CaseReport #AortaEd Postpartum (day 8) acute type B #AorticDissection in 36 y/o♀️ with ACTA2 p.Arg149Cys variant No aortic dilatation (but what's aortic diameter?🧐) Blood pressure managed No preemptive surgery No complication by 1 yr f/up #JACCCaseRep 2026 www.sciencedirect.com/science/arti...
Nanodelivery/Nanoengineering/Nanozyme strategies for skeletal muscle repair & regeneration Tuning inflammation, oxidative stress, muscularization, vascularization, innervation #JNanobiotech 2026 link.springer.com/article/10.1...