It´s #MicroscopyFriday. Here a nice example of Fusarium culmorum invading barley lemma epidermis. Propidium-iodide stains the plant cell wall, ink fungal surface strcutures and wheat germ agglutinin post-invasive fungal cell wall. Overlay of three detectors with CLSM transmission channel.

It´s #MicroscopyFriday. Single cell transformation of barley stomata subsidiary cell. RACB-RIC171 complex (green), mCherry (red), cell wall autofluorescence (light blue). chlorophyll fluorescence (mighnight blue). Have a nice weekend.

It´s #Microscopyfriday. Tobacco expressing the activated barley type I ROP RACB-G15V loses polarity in root hairs and growth isotropically.

‪ #MicroscopyFriday: One of my favourites. Tonoplast marker line of Arabidopsis under attack from Blumeria hordei.

It is #Microscopyfriday. Chevron barley ear tissue stoma (magenta) invaded by Fusarium culmorum hyphae (green, wheat germ agglutinin staining). Note the substomatal vesicle like fungal strcture!

. It´s #Microscopyfriday. Barley epidermial cell under plasmolysis. Type II ROP GTPase marker, GFP-RAC3.

It´s #Microscopyfriday again. Today, I found this on my harddrive. A GFP and barley Bax Inhibitor-1 expressing epidermal cell after single cell transformation of powdery mildew resistant mlo5 barley. Cell-autonomous heterologous compelementaion of susceptibilty allows for fungal sporulation.

I like the #Microscopyfriday idea and gonna post beautiful pictures from my lab. I start with Bipolaris sorokiniana attacking barley epidermis. Inverted picture. Note the nice halo around the appressorium like structure!

‪ If you like to play with structures, here are the links: www.rcsb.org/structure/9T3F www.rcsb.org/structure/9T3E www.rcsb.org/structure/9T3D www.rcsb.org/structure/9T3C www.rcsb.org/structure/28NN

Here is an example of GFP-CARa localization at the extrahaustorial membrane that did not make it into the manuscript but still is nice.