Take a look at our new paper on the structure of the EHD2 that, upon oligomerization, forms rings that remodel the plasma membrane and form caveolae. Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...

It's always a pleasure giving a talk and visiting ETH Zurich @ethz.ch. Fantastic campus with excellent science, thank you for having me !

Went to a wonderful GRC on 3dem and discovered a new GPCR structure!! It has alpha helical resolution and it's an inactive state

No spoilers this time, just the beautiful structure! Mehmet Gul led the work with the dream team of Alice Rossi, Christian Spahn, and Gary Lewin. Enjoy it and have a fantastic day

It's a team effort of the group: Igor, Ulja, Xiaofeng, Artsemi, Patricia, and we thank the funders. The print version will come up in a book on methods in molecular biology, hopefully later this year! Enjoy reading and have a great week!

The book chapter is a set of methods to express your membrane protein of interest, purify vesicles, image them by cryo-ET, and perform subtomogram averaging. As usual, we like to go into details:

The chapter is inspired by our recent paper, where we show that tetrameric and pentameric forms of the serotonin receptor ion channel 5-HT3R were present in the same membrane patches www.embopress.org/doi/full/10....

Building atomic models to cryo-EM maps of huge molecules is intimidating: crowded, uncertain copy numbers, variable resolution ... Fan Liu and team incl us, show how cross-linking MS can be used to build novel models of unknown complexes, like a giant Melbourne virus biorxiv.org/content/10.1...

We have a preprint for you EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

Hi Cryo-EM fellas! What are the pros and cons of the latest cryoFIB milling tools? Any usage experience with Arctis already? Any huge advantages of plasma?

Sooo, does anyone know the actual specs of the newly announced Thermo Fischer Krios G5? Marketing material is wonderful, but what does it actually do ? 😁

S-layer is a very advanced protective layer, optimized by evolution, and understanding its structure is highly important for its potential destruction. It was a team effort and we thank our institutes, colleagues, and funders!

Adria Sourges expressed and co-crystallized the combinations of the domains to get the structures of the interfaces, which finally allowed us to build the correct atomic model, which was validated by mutations. A single mutation at the D2-D2 interface can break the lattice

It is a nice structure but the resolution was not enough to place 6 very similar domains into it. Alex Pak from Colorado performed MD on several of the possible arrangements, checking their stability over time

But it was only a partial construct and in the real bacteria, they form a lattice. Sander Van Der Verren produced pretty tubes, which Kendra Leigh imaged with cryo-ET and got a 7 Å structure by subtomogram averaging.