Watching a video showing (simulated) fireworks sent by a friend ( www.facebook.com/share/v/15u3... ) For some reason.. I keep seeing cladograms in the video 😄😄

14) equitable bioinformatics ecosystem - principles, attributes & enablers? (Need to discuss with @sudattlam @angmiayang for our hospital) (see below post-its and notes for amazing gems💡) 15) wastewater surveillance-useful & (I guess) safe- no patient identity 8/n

2) the partnership between Centre for Pathogen Genomics @TheDohertyInst (headed by Prof @BenjaminHowden ) and IPSN to build sustainable initiatives for pathogen surveillance in many parts of the world 🙏 (some very remarkable experiences including labs in Fiji and Bhutan 🩷) 2/n

This allowed the faster-growing, desiccation tolerant ST22-IV to gain a competitive niche in HCTM, and is now the dominant clone in the hospital (even in 2021). Our next surveillance is 2025, let us see if this clone stays dominant, and if ST6-IV will gain a bigger presence 9/n

On an interesting note: we observed that ST239-III produced smaller, bright banana-yellow clonies, while ST22-IV produced slightly larger, pale yellow colonies 7/n

We selected 4 strains from each surveillance year (no money!) for genome sequencing. Yes clonal change occured; probably after 2013, and before 2017. You can see ST6-IV "stealthily" creeping in abundance from 2005 - 2017 - a clone to look out for in future? 5/n

We wondered when the clonal replacement occured in HCTM. We took a look at antibiogram profiles of MRSAs from 2005, 2009, 2013 and 2017 (thank you @silargi for teaching us this! :) note changes in antibiotic susceptibilities-> the possibility of a clonal change 4/n :

We discovered this clonal replacement in our university hospital (HCTM), when we performed molecular surveillance (SCCmec typing) in 2009, and later in 2017. The change in clones prompted us to perform a "retrospective" surveillance on MRSAs isolated in HCTM from 2005-2017. 2/n