The mature N-terminus is E24, with no N-acetylation. UniProt wishes it were T30, while Gemini really spins a tale to support Q22 as the N-terminal residue. Except residue 22 is an L, not a Q, and the rest of the sequences it uses aren't from EUG1:p.

The mature N-terminus is Q23 after the removal of an ER signal peptide, which is not acetylated but which spontaneously becomes blocked anyway (pyroglutamic acid). UniProt prefers P29 (although it may hedges on Q23 in a quiet voice, depending on how you want to read the PDI1 entry).

The experimentally determined mature N-terminus of HSP60:p is S22, without N-acetylation. UniProt gets it wrong, but Gemini gets it right or wrong, depending on how you ask the question.

The sequence looses its mitochondrial transit peptide, resulting in F27 becoming the N-terminus, far from the evil magics of any N-acetyltransferase. UniProt & Gemini both get this one wrong.

The combination of particulates from fires in northern Canada & Minnesota, with an assist from Washington & Oregon, in combination with a large low pressure system centred just north of the Labrador is resulting in some hazy weather in Ontario & New England. (PM₁₀ & 500 hPa winds)

Canadian forest fire smoke in the north & African dust in the south. (PM₁₀ & 500 hPa winds)

Plenty of dust/sand zooming across the Atlantic all the way to the Caribbean and Gulf of the Mad King. (PM₁₀ & surface winds)

Typhoon Bavi is right on course to make landfall just north of Wenzhou, a city of ~ 10 million people.

#proteomics_101 How would you go about estimating the number of tyrosine kinases necessary to generate this pattern of pY acceptor occupancy in human or mouse KIT:p?

Typhoon Bavi is heading for the north coast of Taiwan & on course for landfall at Wenzhou, China.

And to make all things complete, the trillion dollar djinn produced some thoughts on the significance of a protein sequence having multiple copies of the "Ser-His-Ile-Thr-Thr-Tyr" motif.

#proteomics_101 Yeast ELO3 is an ER type II membrane enzyme that extends very long fatty acids. It has 6 or 7 TM regions, depending on which algorithm you ask. How would you go about determining which is correct & explain why "both" isn't a viable answer?

The trillion dollar goofball managed to come up with a grammatically correct but even more giggle-inducing alternative than any that had occurred to me.

#proteomics_101 Heat Shock (transcription) Factors 1 & 2 are frequently discussed as very similar paralogs. However, HSF1:p seems to use exon 9 as a cyclin-dependent regulatory element via S+phosphoryl acceptors, while HSF2:p does not. Provide the case for further investigation of this observation.

#proteomics_101 Develop a classification scheme for these 8 E2F transcription factors based on their phosphorylation & 3D structure patterns. How does this classification scheme compare with the conventional grouping of these proteins into activators & repressors.

The thing that will usually cause me to bail out of accessing a web site before I even get there.

#proteomics_101 Why does UniProt's list of Gene Ontology terms for yeast ACL4:p include entries associated with mitochondrial outer membrane transport, even though the only known function of this protein is to act as an important chaperone for ribosomal assembly?

Looks like there may be some #aurora tonight in North America!

The Ω pattern that is causing the heatwave in Europe this week. (winds @ 250 hPa)

Things are predicted to be much more moderate, up here in the Winterfell of the Plains.

In the final instalment of the #queso_conundrum, SkyNet tips its hand on the choice of materials for a new Terminator weapons system.

#queso_conundrum These parables are examples of a category error where LLMs fail badly if used to discover new things. They have no info regarding cheese as a structural material, so when asked they generate the appearance of an argument based on misusing info they have, i.e., cheese as food.

#queso_conundrum of the day. I particularly like the storage suggestions at the bottom of the page to show that it is serious about the durability of its suggested Russian cheese.

The markets' ability to accurately price the value of a company has moved solidly from "the wisdom of the crowd" to "the madness of crowds" in just 1 year.

POR1:p uses β-strand transmembrane domains that are only 10 residues long held in place by extensive inter-strand hydrogen bonds. This makes it possible to pack 19 into the 283 residue sequence. This type of TM does not cause any problems for MS-based proteomics: >90% coverage is not unusual.

#queso_conundrum Even though the trillion dollar mechanical Turk believes it has found a solution, there are no words to describe the horror show that would result from using ground cheese (of any type) as cat litter.