Check out this compact 920 nm ultrafast fs laser for 2p imaging of GCaMP, etc.. Mounted on its side like this, it takes up very little space on the table. That's the Axon 920, generously loaned to the course thanks to @ewadorotaz.bsky.social and others at Coherent. It's what we use on our own rigs.

Che-Hang brought the Ventana, and @ianoldenburg.bsky.social brought the holography. All-optical 2p neurophysiology @cshlcourses.bsky.social A great collab. Summer courses are the best.

On Instagram now too... www.instagram.com/pacificoptica/ And LinkedIn... www.linkedin.com/posts/pacifi... Let's keep in touch.

The turnkey Ventana Wide 2-photon imaging system is in service at the CSHL Imaging course. Dr. Che-Hang Yu is happy to share its capabilities. @cshlcourses.bsky.social

Imaging fish at MBL. Field-of-view is 2 mm x 1 mm. 2048 x 1024 pixels. (submicron pixels) with the Ventana system -- the fastest imaging systems over large fields of view. pacificoptica.com/ventana/

We're at the MBL in Woods Hole, MA. Stop on by if you want to try it out for yourself. 2-photon imaging over a 3 mm x 3 mm field-of-view. The most compact mesoscale 2p also has the fastest scanning! pacificoptica.com/ventana/ pacificoptica.com/cousa/

FN > 45 Whatever objective you use, Ventana imaging systems will get more field-of-view out of it, with full NA, and diffraction limited. The best ever for multiphoton / two-photon. And fast! 16,000 - 24,000 lines/s across the full field of view. pacificoptica.com/ventana/

Cool paper from Garret Stuber's lab and colleagues, led by Madelyn Hjort. mPFC-VTA circuit for contingency degredation. doi.org/10.1038/s415... Fig. 3 & 4 used the Cousa objective: **2p imaging + 2p holographic optogenetic stim., deep in brain using microprisms** ! pacificoptica.com/cousa/

All Pacific Optica products are designed in Santa Barbara, California, USA.

Laser scanning on your equipment entails tradeoffs between speed, resolution, and field-of-view. Pick 2 of those 3. Now you can get all 3, with this breakthrough. www.laserfocusworld.com/optics/artic... Thanks @laserfocusworld.bsky.social

Huge field-of-view, fast, high res. two-photon imaging. This technology (paper link below) is available now, in the Ventana products. Sold as complete turnkey systems, or components. pacificoptica.com/ventana/ Installations are happening now at some of the best labs in the world.

An excited customer, sharing photos of the optics they just received. And one of the first Avocado objectives in customer hands. It's a Cousa-class objective, with an NA of 0.6 and a WD of 10 mm, optimized for 2p, and excellent performance out to the edges of the field-of-view. More info soon!

Loving the "CEO eats his own burger/hot dog/..." stuff. Pacific Optica's CEO uses his own Ventana, Diesel2p, and Cousa products. They're the high throughput, turnkey, workhorse systems that power the lab. pacificoptica.com/ventana/ pacificoptica.com/diesel2p/ pacificoptica.com/cousa/

“Because [science] is the most effective way of learning about the physical world, it erodes superstition, ignorance and prejudice, which have been at the root of the denial of human rights throughout history…” -Baron Taverne

“Because scientific knowledge is tentative and provisional, it is the enemy of dogma.” -Baron Taverne

“Because science rejects claims to truth based on authority and depends on the criticism of established ideas, it is the enemy of autocracy.” -Baron Taverne

In 2025, we unlocked new frontiers in multiphoton optics and imaging. In 2026, you can get the best 2p systems you’ve ever seen. Accelerating your work. Higher throughput. Easy to use. Focus on your neuro work, and leave the 2p to PacOp.

Happy Halloween! pacificoptica.com/cousa/

Check out some sweet Ventana Wide + Cousa imaging. pacificoptica.com/ventana/ Live raw data over a 3.2 mm wide field-of-view with 16,000 lines/s scanning and submicron resolution. Demo now at @omibs-mbl.bsky.social Thx @ewadorotaz.bsky.social @mbfbioscience.bsky.social @crisrodtsou.bsky.social !

Scientists explore, measure, gather evidence, figure things out, and share openly. Their work benefits us all. Demo imaging at MBL/Woods Hole and CSHL this summer. Explore further. pacificoptica.com

Put whatever objective you want on the Ventana Wide. It will give you the largest field of view with high speed scanning: eg, 3.2mm x 3.2mm with a 10x. Fast, flexible 2p imaging. Che-Hang Yu is helping MBL courses with this Ventana Wide system pacificoptica.com/ventana/

Just landed in Woods Hole for summer courses: a Full Ventana Wide system. pacificoptica.com/ventana/ with a Cousa pacificoptica.com/cousa/ www.mbl.edu/education/ad... www.mbl.edu/education/ad... Thanks, Coherent! Thanks, MBF! Thanks to course leadership and MBL staff!

Widespread cortical representations of innate behaviors in the mouse. www.biorxiv.org/content/10.1... Using the Diesel2p pacificoptica.com/diesel2p/, they found that "more directed and unilateral grooming movements had greater cortical associated responses than more stereotyped bilateral movements"

No more stitching artifacts. Just image the whole field-of-view, at high resolution, in one pass. The Ventana systems give submicron resolution over huge fields-of-view almost 10 square mm. At high speed. Cover a 3.2 mm x 3.2 mm area in 4k resolution in 0.26 s. pacificoptica.com/ventana/

Explore further. pacificoptica.com/diesel2p/

Animals sense in complex environments, and neuronal responses to olfactory/scent stimuli can be shaped by recent history going back ~ 30 s. Here, Subramanian, Storace, et al. used the Cousa objective pacificoptica.com/cousa/ to neural activity in vivo. doi.org/10.1093/chem... #microscopymonday

Multinucleated giant cells (MNGCs) emerge as ovaries age, and are hard to study. Here, Converse, Pritchard, Duncan et al. used 2p imaging with the Cousa objective pacificoptica.com/cousa/ in their comprehensive study of age-associated MNGCs. #microscopymonday www.biorxiv.org/content/10.1...

There is a bunch more in the paper, including this variation for doubling the phase modulation off of an adaptive optic element. Limited range on your deformable mirror? Not a problem anymore. 8/9

Here's imaging over 9 square mm of cortex in vivo. Resonant scanning at 16,000 lines / s. No stitching at all. 7/9

The approach is diffraction-limited and provides excellent results. 6/9