August issue is out! journals.iucr.org/f/issues/202... On the cover: the crystal structure of LegY, a putative glycosidase effector from Legionella pneumophila, shows high similarity to fungal glucoamylases but shares lower homology with known prokaryotic counterparts doi.org/10.1107/S205...

A new type of article for Acta F: Protocols The purpose of this new category is to provide to the reader procedures that are sufficiently detailed so other researchers can reproduce the described workflow. Find out more in 'Show me how it's done: introducing Protocols': doi.org/10.1107/S205...

We're celebrating 20 years of Acta Crystallographica Section F!🎉🥳 Join us by contributing to our celebratory collection of articles, which is now open for submissions! Find out more at journals.iucr.org/special_issu...

A crystal structure of PLpro exhibits Ubl2 in two distinct conformations: the well characterized closed state, where Ubl2 is positioned near the PLpro domain, and an as yet uncharacterized open state #SARSCoV2 #PapainLikeProteases #Ubl2Domain doi.org/10.1107/S205...

A new crystal structure of ApePCNA1 and the newly solved structure of the ApePCNA2 homotrimer allowed modeling of the ApePCNA1–ApePCNA2–ApePCNA3 heterotrimer #AeropyrumPernix #DNAReplication #ProliferatingCellNuclearAntigens doi.org/10.1107/S205...

The crystal structure of GABA aminotransferase from A. thaliana and structural comparison supports the conclusion that it belongs to the class III aminotransferase family #ArabidopsisThaliana #CrystalStructure #GABAAminotransferase doi.org/10.1107/S205...

The molecular interactions of the insomnia drug vorenorexant with human orexin receptors were analysed through crystal structure analysis and docking simulations #Vornorexant #OrexinReceptors #CrystalStructure doi.org/10.1107/S205...

This structure of macrophage migration inhibitory factor is a contribution to our cross-journal Early Career Scientists in Structural Science special issue. Find out more at journals.iucr.org/early-career... #SSGCID #MacrophageMigrationInhibitoryFactor doi.org/10.1107/S205...

July issue is out now! journals.iucr.org/f/issues/202... This month's cover star is the type VI secretion system adaptor protein Tla3 from Pseudomonas aeruginosa strain UCBPPPA14. Find out more about this structure at doi.org/10.1107/S205...

The structure of spectrin-like repeat 24 of human dystrophin is in excellent agreement with the AlphaFold model, supporting AlphaFold modeling of all 24 dystrophin spectrin-like repeats #Dystrophin #SpectrinLikeRepeat #DuchenneMuscularDystrophy doi.org/10.1107/S205...

The structure of a complex between human RAGE ectodomain and S100A6 variant Y84C provides a possible model for S100 covalent homodimerization that is fully compatible with RAGE binding #S100Proteins #Cysteines #CovalentHomodimerization doi.org/10.1107/S205...

The first structure of a cold-active arginase reveals a modest reduction in structural rigidity associated with cold adaptation #ColdActiveEnzymes #Arginases #XRayCrystallography doi.org/10.1107/S205...

Acetylation-mimicking K214Q and K216Q variant structures of Escherichia coli glucokinase showed that acetylation mimics impaired activity and produced domain shifts #Glucokinases #LysineAcetylation #AcetylationMimickingMutations doi.org/10.1107/S205...

Structural and enzymatic characterization of an Asp49 phospolipase A2 (PLA2) from Lachesis muta expands our understanding of structure–function relationships among viperid PLA2s #SnakeVenomPhospholipaseA2 #LachesisMuta #ProteinOligomer doi.org/10.1107/S205...

The June issue is out! journals.iucr.org/f/issues/202... The cover illustration - Plasmodium vivax macrophage migration inhibitory factor - is from an article forming part of our Early Career Scientists in Structural Science special issue. Find out more and submit: journals.iucr.org/early-career...

New issue is out! journals.iucr.org/f/issues/202... On the cover: the atomic structure of human dystrophin spectrin-like repeat 24 determined at 2.14 Å resolution journals.iucr.org/f/issues/202...

Modifying the bacterial strain led to cleaner bacterial expression of a synthetic BRIL antibody that facilitates protein structure determination via both crystallography and cryo-EM #CryoEM #XRayCrystallography #BRIL doi.org/10.1107/S205...

High-resolution structures of α-bCA2 with sulfonamide derivatives provides experimentally supported information defining their binding mode and supporting inhibitor optimization #CarbonicAnhydraseII #SulfonamideInhibitors #XRayCrystallography doi.org/10.1107/S205...

An acylated GLP-1/GIP analogue peptide forms a uniquely porous spiral crystal structure composed of clockwise-ascending monomers in a square pattern #GLP1 #Peptides #Crystallization doi.org/10.1107/S205...

A comprehensive survey catalogues all pentatricopeptide repeat protein crystal structures, analysing their crystallographic properties, structural features and contributions to understanding function #PentatricopeptideRepeatProteins #RNARecognition doi.org/10.1107/S205...

Our April issue is out now! journals.iucr.org/f/issues/202... Crystallization was investigated as a preparative strategy for a GLP-1/GIP lipopeptide and led to what is believed to be the first reported crystal structure of an unbound GLP-1/GIP analogue doi.org/10.1107/S205...

The structure of endo-β-N-acetylglucosaminidase was determined at 1.8 Å resolution, revealing a unique spatial arrangement of domain IV, which is suggested to contribute to substrate recognition #EndoNAcetylglucosaminidases #GH85 #ComplexNGlycans doi.org/10.1107/S205...

The structure of a chimeric efflux pump was determined by cryoEM under apo and kanamycin-supplemented conditions and adopted symmetric-like and asymmetric conformations #PseudomonasAeruginosa #DrugEffluxTransporters #MembraneProteins doi.org/10.1107/S205...

MBP fusion is an effective and generalizable approach to enable crystallographic studies of engineered binding proteins that are otherwise difficult to crystallize #Monobody #Aggregation #MaltoseBindingProtein doi.org/10.1107/S205...

This report of the structure of a carbon-nitrogen hydrolase doi.org/10.1107/S205... is the first contribution to the Early Career Scientists in Structural Science special issue! Find out more, join in and submit at journals.iucr.org/early-career...

Here's our March issue: journals.iucr.org/f/issues/202... On the cover: the structure of the chimeric RND-type multidrug-efflux pump protein MexBYB was determined by cryoEM under apo and kanamycin-supplemented conditions journals.iucr.org/f/issues/202...

This structure of a tRNA acceptor-stem mimic demonstrates the use of bromine and phosphorus anomalous scattering for RNA structure determination #Crystallography #AnomalousScattering #NucleicAcids doi.org/10.1107/S205...

Sample preparation from α- and β-crustacyanin pigments purified from ex vivo material from the American lobster Homarus americanus for X-ray crystallography and cryo-electron microscopy studies #Astaxanthin #Crustacyanin #Absorption doi.org/10.1107/S205...

The highest resolution DNA-free TaXPD structure to date uniquely resolves loop 510–514 in the long-form, DNA-free TaXPD model #XRayCrystallography #Helicases #Archaea doi.org/10.1107/S205...

Potential 14-3-3 binding sites in sirtuins were identfied and the interactions of peptides representing phosphorylation sites in sirtuin 3 and sirtuin 1 with 14-3-3 proteins were characterized #Sirtuin #Phosphopeptides #Regulation doi.org/10.1107/S205...