Amazingly, translation of the majority of FMRP high-binding mRNAs was increased when we inhibited Me31B through expression of a dominant-negative transgene - only upon FMRP depletion, showing that excessive repression was responsible for diminished translation when FMRP protection was lost.

We found that FMRP didn’t do this job alone. Using a single molecule FISH based screening assay in developing egg follicles, FMRP-driven translation sites required the presence of partner stress granule-linked proteins whose functions remain fairly mysterious.

Using iiCLIP-seq, we found that almost all (94%) of FMRP high-binding target mRNAs were also strongly bound by Me31B, and both proteins bound to mRNA in an exquisitely length-dependent manner.

We now show that FMRP acts to protect long mRNA targets from repression by the central repressive factor Me31B/DDX6, which is a core RNP remodeling protein that drives formation of P-bodies.

Making my voice heard at the #nokings protest in Vancouver!

Hi Bluesky 🦋👋. Really don’t have time for fascist enabling social media platforms at the moment