AIC at Janelia

@aicjanelia

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AIC at Janelia@aicjanelia· 16d

Continuing our discussion of super-resolution techniques, our morning lecture at #IALSJanelia focused on analysis of localization microscopy data. Tessellation of this iPALM dataset reveals not only the boundary of the focal adhesion, but also internal structure within.

Visualization of a tessellation of point coordinates. White lines indicate the boundaries of the tessellation, while color indicates the area of the tessellation region (blue = large region, yellow = small region).

AIC at Janelia@aicjanelia· 17d

Today is super-resolution day at #IALSJanelia! We're spending the afternoon at the microscope exploring these beautiful tubulin networks🔬 Images by @harikrushnan.bsky.social

AIC at Janelia@aicjanelia· 20d

How does resolution impact colocalization? This afternoon at #IALSJanelia participants are exploring the impact of imaging modality on measurements of co-occurrence and correlation.

A two-panel microscopy image. Both panels show mitochondria labeled for COX8 (green) and TOMM20 (magenta). The left panel shows an image taken on a widefield microscope, and most pixels are white (a merge of magenta and green). The right panel shows an image taken using lattice SIM.

AIC at Janelia@aicjanelia· 20d

We are grateful to Paul Tillberg for stopping by to give #IALSJanelia 2026 participants a guest lecture on expansion microscopy, including a hands-on #ExM demonstration!

A photo of a conference room where attendees are sitting at rows of tables. At the front of the room, a man sits alone at a separate table with a document camera. A view of the samples he is manipulating is projected on the screen behind him.

AIC at Janelia@aicjanelia· 21d

This afternoon's imaging lab at #IALSJanelia is all about subcellular structures, highlighted here by myosin (white) and actin (orange). These membrane ruffles were captured on our lattice light-sheet microscope by @cmhobson.bsky.social

AIC at Janelia@aicjanelia· 22d

During our first analysis lab at #IALSJanelia 2026, participants are exploring #TrackMate. How do tracking challenges (and advantages) change when you image different parts of the cell? Do you get the same results from different labels?

AIC at Janelia@aicjanelia· 23d

For our first live cell imaging lab of #IALSJanelia 2026, we're exploring the migratory behavior of neutrophil-like HL60 cells. Confocal microscopy by @anjanja1024.bsky.social 🔬

AIC at Janelia@aicjanelia· 23d

We're exploring Fourier transforms at #IALSJanelia. In addition to applying them computationally in #Fiji, we're performing them optically with the help of a @thorlabs.bsky.social optics kit run by @cmhobson.bsky.social!

A photograph of optics equipment on a table. The Fourier pattern is projected onto a white piece of paper, while the image pattern is seen in the upper right corner of the photo.

AIC at Janelia@aicjanelia· 23d

This morning at #IALSJanelia, we're jumping into image analysis with #FIJI / #ImageJ! Our practical exercises on image filtering include comparisons of denoising approaches.

A man sitting at a table looks at his laptop. The laptop shows an image of a clown that has been overlaid with noisy speckles.

AIC at Janelia@aicjanelia· 24d

This morning at #IALSJanelia we're getting started with some microscopy fundamentals in a lecture by @cmhobson.bsky.social 🔬

A photo of people in a conference room. A man stands at the front next to a large projector screen. The screen shows a presentation slide on image formation.

AIC at Janelia@aicjanelia· 25d

We're kicking off #IALSJanelia 2026 with a keynote lecture by @scopeshifu.bsky.social. Our teaching team is excited to get to know all our participants and start two weeks of talking about microscopy! 🔬

AIC at Janelia@aicjanelia· Jul 7

Despite its nebula-like qualities, this image is from the microscopic rather than astrophysical scale. This adherent cell was labeled for focal adhesions (blue structures) and actin (orange fibers). It is one of the many samples the team is hard at work preparing for #IALSJanelia 2026!

An image taken on a microscope of a single adherent cell. Two images were combined in this visualization: an image taken with widefield illumination (orange colors) and an image taken using TIRF (blue colors). Together these colors highlight fibers of actin and elongated focal adhesion structures.

AIC at Janelia@aicjanelia· Jul 7

We enjoyed having Wenting Zhao and team here to take data on our iPALM system with @anjanja1024.bsky.social and wish them happy data analyzing!

A photo of a group of women standing in front of a staircase.

AIC at Janelia@aicjanelia· Nov 7

One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬

A photo of a coffee mug. The mug is labeled with writing that says "hhmi | janelia Research Campus." The drink in the mug has the words "Insight From Imaging 2025" printed on the foam.

AIC at Janelia@aicjanelia· Nov 7

For our final lecture at #InsightFromImaging 2025, @jesseaic.bsky.social is discussing electron microscopy and correlative approaches, including results from our FIB-SEM system, which can achieve 4 nm resolution in 3D!

AIC at Janelia@aicjanelia· Nov 6

We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth. Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE

A 3D rendering of microscopy data. Fluorescently labeled tubulin is shown in green, while second harmonic generation is shown in magenta.

AIC at Janelia@aicjanelia· Nov 6

Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.

A microscopy image with text annotation. The left side of the image is labeled "System Correction Only" and shows blurry and aberrated mitochondria. The right side of the image is labeled "Adaptive Optics + Deconvolution" and shows a clearer image of mitochondria.

AIC at Janelia@aicjanelia· Nov 5

This morning at #InsightFromImaging we focused on particle tracking. After a lecture by @jesseaic.bsky.social, participants split into groups to use #TrackMate to track our HL60 migration data from yesterday, followed by presentations on their results 📈

A collage of photos, which show people analyzing images and giving presentations.

AIC at Janelia@aicjanelia· Nov 4

Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.

AIC at Janelia@aicjanelia· Nov 4

Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.

A photo of a classroom. A man stands at the podium next to a projector screen. The audience sits at tables in front of the screen.

AIC at Janelia@aicjanelia· Nov 3

For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?

A microscopy image of a cell labeled for actin (magenta) and paxillin (green). The left half of the image is taken in widefield mode, while the right half is taken in confocal mode. The scale bar is 10 um.

AIC at Janelia@aicjanelia· Nov 3

This morning at #InsightFromImaging we jumped into hands on image analysis activities, including comparing the pros and cons of imaging denoising approaches

A photo of a classroom. There is a projector screen at the front of the room, and participants sit at tables with laptops performing image analysis tasks.

AIC at Janelia@aicjanelia· Nov 2

We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬

A photo of a man giving a presentation. He is standing in front of a projector screen. In the front of the photo, the audience sits at long tables.

AIC at Janelia@aicjanelia· Oct 31

Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.

AIC at Janelia@aicjanelia· Oct 27

Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments 🔬

A microscopy image of a cell stained for actin (magneta color) and focal adhesions (green color).

AIC at Janelia@aicjanelia· Oct 10

Today we're wrapping up a flatworm imaging project on our SiMView microscope. We've enjoyed having @kinglhung.bsky.social and team from the lab of @ardemp.bskyverified.social here and look forward to working with them on analyzing their images!

A photo of two rows of people. The first row of people is sitting on bench while the second row stands behind them, in front of floor to ceiling windows. Outside the windows is a fall scene with trees.

AIC at Janelia@aicjanelia· Sep 26

Today we're wrapping up 3 weeks of zebrafish imaging on our MOSAIC system. We've enjoyed having Lisa and Miray of @annamolofskylab.bsky.social here and wish them safe travels home!

A photo of a group of people standing in front of scientific equipment.

AIC at Janelia@aicjanelia· Sep 5

Today we're wrapping up an iPALM visit with @anirban-banerjee.bsky.social, @souravsgw.bsky.social, and team. Wishing them safe travels home!

A photo of a group of people. A group stands in front of the wall, while two women sit in chairs in front of them.

AIC at Janelia@aicjanelia· Aug 8

We've enjoyed having Marise and Samara from the lab of @melaniedwhite.bsky.social here to image quail embryos on our MOSAIC system. We wish them safe travels home and happy data analyzing!

A photo of a group of five people standing in a room filled with scientific equipment.

AIC at Janelia@aicjanelia· Jul 25

We are wrapping up a project imaging cancer cell spheroids on our SiMView system today. We wish Elizabeta Belova of @temple.edu happy data analyzing and safe travels home!